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Vairam, N.
- Frequency and Spectrum of Chlorophyll Mutations in Greengram [Vigna radiata (L.) Wilczek]
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Affiliations
1 Department of Plant Breeding and Genetics, Agricultural College and Research Institute (T.N.A.U.), Madurai (T.N.), IN
1 Department of Plant Breeding and Genetics, Agricultural College and Research Institute (T.N.A.U.), Madurai (T.N.), IN
Source
Asian Journal of Bio Science, Vol 9, No 2 (2014), Pagination: 204-207Abstract
Greengram [Vigna radiata (L.) Wilczek], popularly known as mungbean, is third most important pulse crop of India. Mungbean is a cheap source of dietary protein for the poor, with high levels of folate and iron compared with many other legumes. Variability is low available in mungbean and hence, to replace conventional breeding, mutation breeding has gained its momentum. Induced mutagenesis thus seems to be an ideal methodology for the induction of desirable genetic variability. Chlorophyll mutations, an important index in the estimation of induced genetic changes in mutagen treated population are most widely employed for assessing the potentialities of mutagens in creating genetic variability. An investigation was carried out in two mungbean genotypes CO (Gg) 7 and NM 65 treated by two mutagens viz., gamma rays and ethyl methane sulphonate. A wide range of chlorophyll mutations was observed and scored in M2 generation. The highest frequency rate was noted at 300 Gray and 10 mM on M1 plant basis and M2 seedling basis in both the genotypes. The mutant chlorina and xantha occurred in all the treatments of gamma rays and EMS at higher proportions.Keywords
Greengram, Chlorophyll Mutation, EMS, Gamma Rays, VariabilityReferences
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- Screening of M3 Mutants for Yellow Vein Mosaic Virus Resistance in Greengram [Vigna radiata (L.) Wilczek]
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Authors
Affiliations
1 Department of Plant Breeding and Genetics, Agricultural College and Research Institute (T.N.A.U.), Madurai (T.N.), IN
2 Department of Plant Pathology, Agricultural College and Research Institute (T.N.A.U.), Madurai (T.N.), IN
1 Department of Plant Breeding and Genetics, Agricultural College and Research Institute (T.N.A.U.), Madurai (T.N.), IN
2 Department of Plant Pathology, Agricultural College and Research Institute (T.N.A.U.), Madurai (T.N.), IN
Source
International Journal of Plant Sciences, Vol 11, No 2 (2016), Pagination: 265-269Abstract
Greengram [Vigna radiata (L.) Wilczek] is a cheap source of dietary protein for the poor, with high levels of folate and iron compared with many other legumes. Mungbean yellow mosaic virus is also one of the destructive viral disease affecting yield potential of greengram both quantitatively and qualitatively. Induced mutations, have offered a single and short alternative to conventional breeding including isolation, screening, selection and testing generation after generation. An investigation was carried out in two mungbean genotypes viz., CO (Gg) 7 and NM 65 treated by two mutagens viz., gamma rays at the doses of 300, 400 and 500 Gy and EMS treatments of 10, 20 and 30 mM. The trial was conducted in the research farm of Agricultural College and Research Institute, TNAU, Madurai during Kharif season 2013. The M2 generation was raised as individual M1 plant basis. The treated and control populations of M2 generation were carefully screened for pod shattering resistance. The yellow vein mosaic virus disease (YMV) incidence was recorded for all the plants in M3 generation for the selected 22 mutants. Based on field scoring, the mutants viz., M5, M18, M26, M46, M54, M58, M70, M71, M92 and M98 were identified as yellow vein mosaic virus resistant mutants. The mutants which showed field resistance were checked for YMV resistant gene using the gene specific primer ISSR811. Five mutants confirmed with marker studies. These resistant mutants have been forwarded to next generation for further yield performance.Keywords
Mungbean, Induced Mutation, Yellow Vein Mosaic Virus, Scoring, Mutants, Marker.References
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